Is folklore use of Euphorbia helioscopia devoid of toxic effects?

Saleem, Uzma; Ahmad, Bashir; Ahmad, Mobasher; Erum, Alia; Hussain, Khalid; Irfan Bukhari, Nadeem

Objective: To evaluate acute toxic effects ofEuphorbia helioscopiain order to assure the safety and usefulness of herbal remedy. Materials and methods: The Organization for Economic Cooperation and Development (OECD) for chemical testing guidelines No. 425 for acute oral toxicity testing were followed in this study. Mice were divided into three groups (n = 5). Group I served as control. Groups II and III were administered methanol extract ofE. helioscopialeaves and latex orally at dose of 2000 mg/kg, respectively. Then, all the animals were observed for two weeks. Blood sampling was done by cardiac puncture after 14 days from each group for biochemical analysis. Histopathology was performed to find out any microscopic lesion in vital organs. Results and discussion: LD50was found greater than 2000 mg/kg. There was decrease in cholesterol, triglycerides, LDL and VLDL levels of latex and leaves with methanol extract-treated animals, with respect to control indicating plant’s hypolipidemic effect. On macroscopic examination, no lesions were found on vital organs, such as liver, heart and kidney; and normal architecture was observed on microscopic examination. Conclusion: On the basis of results, it was concluded that methanol extract ofE. helioscopialeaves and latex were devoid of toxic effects in acute toxicity study.

EUPHORBIA; TOXICITY testing; ORGANISATION for Economic Co-operation & Development; BLOOD sampling; HISTOPATHOLOGY; CHOLESTEROL; TRIGLYCERIDES

Drug & Chemical Toxicology, 2016, Vol 39, Issue 2, p233

0148-0545

Academic Journal

10.3109/01480545.2015.1092040