Laserpuncture induction and administering Vitamin E as an opulent antioxidant may improve sperm quality. This study aimed to increase rat reproductive potential by inducing laserpuncture and adding vitamin E to their feed. The experimental study adopted a post-test in a completely randomized design in male albino Wistar rats (Rattus norvegicus), with four groups (Group A to D): Group A: the control group (without vitamin E or Laserpuncture), Group B: Administered with vitamin E, Group C: Treated using Laserpuncture induction, and Group D: Treated using Laserpuncture induction and administered with Vitamin E and five replicates. The rat reproductive potential parameters included testosterone levels, Gonado Maturity Stage (GMS), Gonado somatic Index (GSI), and concentration, motility and viability of sperm. Induction of Laserpuncture and administered with Vitamin E influenced testosterone hormone levels, motility viability, and the concentration of male rat's sperm (P 0.897) with mature gonads, and GMS gave the most mature V stage for all treatments. This study concluded that the combination of Laserpuncture induction and administration with Vitamin E had increased the reproductive potential of male albino Wistar rats in terms of testosterone levels and sperm quality.