We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Gold nanoparticles improve the embryonic developmental competency of artificially activated mouse oocytes.
- Authors
Muhammad, Tahir; Jamal, Muhammad Ameen; Ashraf, Muhammad; Zafar, Nosheen; Shahzadi, Shamaila; Maqbool, Tahir; Hadi, Faheem; Riaz, Amjad
- Abstract
Currently, artificial oocyte activation has attracted wide attention in assisted reproduction due to extensive range of applications, particularly in somatic cell nuclear transfer and deriving pluripotent stem cell lines and it is the unique model to determine the role of paternal genome. Numbers of artificial activating agents have been used extensively to induce the oocytes activation; however, embryos developmental competency of artificially activated oocytes is still very low. In the present study, we determined the functional impact of strontium chloride supplementation with gold nanoparticles (AuNPs) in artificial oocytes activation and subsequent embryonic development. Oocytes were activated artificially in the culture medium containing 250 nM AuNPs with constant concentration of strontium chloride 10.00 mM. We found that adding 250 nM AuNPs with constant concentration of strontium chloride (10.00 mM for 3 hr) in culture medium improves the proportion of embryos reaching to the morula and blastocyst stages from 61.00% and 42.00% (controls) to 75.00% and 58.00% (250 nM AuNPs), respectively. In addition, foster mothers receiving AuNPs-treated embryos showed more implantation percentage and pregnancy rate relative to females received control embryos. Finally, embryos treated with 250 nM AuNPs concentration showed no toxic effect in term of blastocyst development. Collectively, our findings suggest the potential role of AuNPs in early embryonic development for mouse oocytes activated artificially and provide new insights in the field of animal biotechnology and assisted reproduction in humans.
- Subjects
GOLD nanoparticles; VETERINARY embryology; TRANSPLANTATION of cell nuclei; PLURIPOTENT stem cells; PARTHENOGENESIS in animals; STRONTIUM chloride
- Publication
Veterinary Research Forum, 2021, Vol 12, Issue 4, p415
- ISSN
2008-8140
- Publication type
Academic Journal
- DOI
10.30466/vrf.2020.119759.2829