Phenotypic and Genotypic Assortment of Polyhydroxyalkanoates Producing Bacteria from Rumen Flora of Domesticated Animals in Pakistan.

Rafique, A.; Mahmood, M. S.; Ashraf, A.; Nasir, S.; Kamran, M.; Jamil, N.; Jabeen, F.; Sultana, T.; Sultana, S.; Khan, JA; Luqman, M.; Shamim, A.

In plastic industry, a lot of attention has been gained by Polyhydroxyalkanoates (PHAs) owing to their environment friendly nature and 100% biodegradable, so they are called as "green plastics". Bacterial strains able to produce PHAs under veterinary field are reported and rumen microflora was exploited to check its potential for the PHAs production. From the rumen of domestic cattle samples were collected followed by isolation of PHA producing bacteria. Biochemical and molecular characterization of isolates was done through API 20E kit and polymerase chain reaction (PCR), respectively. In order to confirm the genetic diversity, sequencing of 16S rRNA gene, ribotyping and phylogenetic analysis was performed. A deeper insight of PHAs biopolymer by Fourier transform infrared spectroscopy (FTIR) revealed the functional groups. Pseudomonas aeruginosa (P. aeruginosa) was isolated and biochemically confirmed through API 20E. PCR further confirmed P. aeruginosa through amplification of 16S rRNA gene. Sequenced data of 16S rRNA (accession# MN625895) analyzed through ribotyping and phylogenetic linkages proved all the isolate belongs to P. aeruginosa. PHAs were purified from enriched nutrient broth with rumen mixture by using sodium hypochlorite confirmed through FTIR spectroscopy. Complex accumulation of bacterial biomass showed -C=O, C-H and -CH3 functional groups. Bacterial biomass (PHAs) in enriched carbon medium can be used as bioplastics for domestic purpose with less harm. It will be a better alternative to chemically synthesized plastic to reduce environmental hazards.

PAKISTAN; BIODEGRADABLE plastics; POLYHYDROXYALKANOATES; DOMESTIC animals; FOURIER transform infrared spectroscopy; BOTANY; POLYMERASE chain reaction; PSEUDOMONAS aeruginosa

Pakistan Veterinary Journal, 2020, Vol 40, Issue 1, p88

0253-8318

Academic Journal

10.29261/pakvetj/2019.124