COX-mediated Regulation of Multiple Organ Damage by Betulin Treatment in Okadaic Acid-induced Alzheimer Rat Model.

BOZKURT, Ahmet Sarper; YILMAZ, Şenay Görücü

Objective: Alzheimer's Disease (AD) is a progressive neurodegenerative disease. Cyclooxygenases (COXs) are essential in the inflammatory and regenerative processes of AD. This study aims to show that Betulin, a natural phytochemical (triterpene), is a candidate for COXmediated correction of multiple organ damage of AD. Materials and Methods: In this study, the effects and treatment potential of Betulin were investigated in the kidney, heart, and small intestine tissue in genetic, and histological contexts in an okadaic acid-induced rat AD model. A total of 36 Wistar albino male rats were included in the study. Cyclooxygenase 1 (COX-1) and Cyclooxygenase 2 (COX2) gene expressions were investigated by quantitative real-time PCR (qRT-PCR) in kidney, heart, and small intestine tissues. COX-1 and COX-2 proteins in tissues were analyzed by immunohistochemistry. Results: COX-1 and COX-2 genes were detected to be overexpressed in the AD model. The expression of both genes was increased in the AD model and decreased after betulin treatment. Histological scores showed a strong positive effect of Betulin on the kidney, while it was relatively less effective on the heart and small intestine tissue. Conclusions: In treating organ damage in AD, COXs can be inhibited by Betulin and may be effective in functional recovery.

BETULIN; LABORATORY rats; ANIMAL models of Alzheimer's disease; CYCLOOXYGENASE 2; ALZHEIMER'S disease; CYCLOOXYGENASES; NEURODEGENERATION; PHYTOCHEMICALS; SMALL intestine; IMMUNOHISTOCHEMISTRY

Online Turkish Journal of Health Sciences (OTJHS) / Online Türk Sağlık Bilimleri Dergisi, 2024, Vol 9, Issue 1, p73

2459-1467

Academic Journal

10.26453/otjhs.1405878