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- Title
Tumor necrosis factor receptor-associated factor 5 enhances perianal fistulizing Crohn's disease through epithelial–mesenchymal transition.
- Authors
Sun, Xiaomei; Gao, Hairui; Lu, Lu; Wang, Qianqian; Li, Youran; Gu, Yunfei
- Abstract
Objective: Crohn's disease (CD) is a chronic inflammatory condition of the bowel that remarkably impairs a patient's quality of life and often has a poor prognosis. Perianal fistulizing CD (PFCD) is one of the most common parenteral symptoms of CD and a huge challenge for the management of this illness. This study aimed to elucidate the molecular mechanisms underlying PFCD and identify potential biomarkers to advance our understanding and management of this condition. Material and Methods: Transcriptome sequencing was performed using the control and PFCD groups to investigate the mechanisms of PFCD development. The expression of tumor necrosis factor receptor-associated factor 5 (TRAF5), nuclear factor-kappa B (NF-κB), and interleukin 13 (IL-13) messenger ribonucleic acid (mRNAs) was detected by quantitative polymerase chain reaction (qPCR). Pathological morphology was observed using hematoxylin and eosin staining. The expression of TRAF5, Epithelial Cadherin (E-cadherin), Snail family transcriptional repressor 1 (SNAIL1), and vimentin protein was detected by immunohistochemistry. Following the knockdown of TRAF5 in human tumor-29 (HT-29) cells, the effects on cell proliferation and migration were assessed using the cell counting kit-8 and Transwell assays. The expression levels of crucial markers were analyzed by qPCR, Western blot, and immunohistochemistry. Results: Transcriptomic sequencing revealed a significant upregulation of TRAF5 in the PFCD group, accompanied by elevated mRNA levels of NF-κB and IL-13 compared with those in the control group. In addition, the PFCD group exhibited increased expression of TRAF5, SNAIL, and vimentin and marked reduction in E-cadherin levels, indicating that PFCD may facilitate epithelial–mesenchymal transition (EMT). Knocking down TRAF5 in HT-29 cells reduced cell proliferation and migration; inhibited NF-κB and IL-13 mRNAs, SNAIL1, and vimentin levels; and promoted E-cadherin levels. Conclusions: The development of PFCD was associated with EMT, and TRAF5 was a key gene of PFCD. Knocking down TRAF5 alleviated the EMT promotion of PFCD, indicating that TRAF5 drove the development of PFCD through EMT.
- Subjects
PROTEIN metabolism; ANAL fistula; NF-kappa B; CELL migration; CROHN'S disease; EPITHELIAL-mesenchymal transition; QUALITATIVE research; POLYMERASE chain reaction; CELL proliferation; GENE expression; MESSENGER RNA; IMMUNOHISTOCHEMISTRY; TUMOR necrosis factor receptor-associated periodic syndrome; GENE expression profiling; MICROBIOLOGICAL assay; VITAMINS; STAINS & staining (Microscopy); COMPARATIVE studies; BIOMARKERS; SEQUENCE analysis; INTERLEUKINS; TUMOR necrosis factors
- Publication
CytoJournal, 2024, Vol 21, p1
- ISSN
0974-5963
- Publication type
Academic Journal
- DOI
10.25259/Cytojournal_148_2024