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- Title
Disminución de la inmunorreactividad a la subunidad β2 del receptor GABA<sub>A</sub> en un modelo de autismo en ratas.
- Authors
Puig-Lagunes, Ángel A.; Villada, Frida S.; Morgado-Valle, Consuelo; Beltran-Parrazal, Luis; Chi-Castañeda, Lizbeth D.; López-Meraz, María L.
- Abstract
Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the brain, and activation of GABA type A (GABAa) receptors mediates rapid inhibitory actions. Numerous studies have shown that individuals with autism spectrum disorder (ASD) exhibit abnormalities in the expression of GABAa receptors in several brain areas. In addition, animal models of ASD have suggested alterations in GABAergic neurotransmission and dysregulation of the balance between inhibitory and excitatory systems. objective: We investigated the immunolabeling of GABAa receptor β2 subunit (GARB2) in the hippocampus, the amygdala, and the thalamus of infant rats prenatally exposed to valproic acid (AVP) as a model of ASD. Methods: Pregnant female rats were injected with AVP (600mg/Kg, i.p.) on embryonic day 12; control rats were injected with saline (SS group). On postnatal day 14, rats from both groups were anesthetized, transcardially perfused with 0.9% NaCl and 4% paraformaldehyde, and sequential coronal brain slices (40µ m thickness) were obtained. Immunohistochemistry was performed to detect GARB2, and the relative optical density (OD) of immunoreactivity was analyzed. results: Our data showed a statistically significant decrease in GARB2 immunoreactivity in the lateral amygdaloid nucleus and the ventral and lateral thalamic nuclei of AVP group when compared to the SS group. No statistically significant differences were found in the hippocampus. conclusion: Our findings suggest that prenatal exposure to AVP reduces GARB2 immunoreactivity in limbic brain regions involved in social-emotional behavior, consistent with previous reports in individuals with ASD. These findings support for the involvement of the GABAergic system in the pathogenesis of ASD.
- Subjects
BIOLOGICAL models; AMYGDALOID body; AUTISM; HYPERTONIC saline solutions; BRAIN; DESCRIPTIVE statistics; IMMUNOHISTOCHEMISTRY; THALAMUS; MICE; ANIMAL experimentation; VALPROIC acid; HIPPOCAMPUS (Brain); ASPERGER'S syndrome; DRUGS; COMPARATIVE studies; CELL receptors; GABA; SALT; NEUROTRANSMITTERS
- Publication
Archivos de Neurociencias, 2024, Vol 29, Issue 3, p67
- ISSN
1028-5938
- Publication type
Academic Journal
- DOI
10.24875/ANC.M24000002