Results
Title

IN VITRO TESTING OF A POLYLACTIC POLYMER SYNTHESIZED FROM WHEY.

Authors

DREANCĂ, ALEXANDRA; POPESCU, RADU A.; NEAGU, AMALIA; ENACRACHI, GEORGE; CIMPOEŞ, IULIA; FIŢ, NICODIM; MOLDOVAN, MARIOARA; DUMITRESCU, LAURA SILAGHI; JURJ, ANCA; BERINDAN-NEAGOE, IOANA; MARCUS, IOAN

Abstract

The main purpose of this research is in vitro biocompatibility testing of a polylactic acid polymer (PLA) synthesized by the condensation of lactic acid separated from whey (Zonar). The experimental PLA obtained was characterized by: Scanning Electron Microscopy which pointed a homogeneous microstructure of the polymer without a separation phase and differential scanning calorimetry (DSC) analysis which indicated a melting point of 1740C of the biomaterial. The cytotoxicity test for experimentally obtained polymer was performed according to ISO 10 993 -123. Six solutions of different concentrations were prepared (30 mg/ml, 20 mg/ml, 10 mg/ml, 5 mg/ml, 2.5 mg/ml and 1.25 mg/ml) and were tested on a fibroblast culture at 48 hours using MTT cell viability and proliferation assay. At 48h the obtained polylactic polymer showed good compatibility with an IC 50 of 64.82 mg/ml. The results showed that the cytotoxicity level can be influenced by the period of time in which the extract acts on the cell culture.

Subjects

POLYLACTIC acid; POLYMERS; WHEY; BIOCOMPATIBILITY; LACTIC acid; MICROSTRUCTURE

Publication

Studia Universitatis Babes-Bolyai, Chemia, 2018, Vol 63, Issue 2, p93

ISSN

1224-7154

Publication type

Academic Journal

DOI

10.24193/subbchem.2018.2.09