Results
Title

A Study of Genotypic Characterization of ESBL and MBL Genes of β-Lactamase Producing Pseudomonas aeruginosa in Various Clinical Samples.

Authors

Rengaraj, Ramya; Muninathan, Natarajan; Alagiri, Sivaranjini; Suresh, Arumugam

Abstract

Pseudomonas aeruginosa, a Gram-negative bacterium, presents a substantial challenge in healthcare due to its adaptability and resistance. This study delves into its genotypic characteristics, focusing on ESBL and MBL genes. The prevalence of P. aeruginosa in nosocomial infections, the research aims to decipher resistance mechanisms crucial for tailored interventions. The study includes 170 nonrepetitive clinical samples with protocols. Antibiotic susceptibility testing reveals diverse resistance patterns. Molecular detection of ESBL and MBL genes involves DNA isolation, PCR amplification, and gel electrophoresis. The study examined 170 P. aeruginosa samples, revealing gender-specific variations with 65.91% male and 34.09% female isolates. Antimicrobial testing displayed resistance in Ceftazidime (59%) and Ciprofloxacin (48%), while Ticarcillin-clavulanic acid showed promising sensitivity (58%). Molecular identification unveiled diverse resistance genes across sample types, emphasizing genetic complexity. The study underscores the urgency for targeted therapeutic interventions and novel antimicrobial strategies against P. aeruginosa infections. As antimicrobial resistance complexities persist, this research guides efforts toward a profound understanding of clinical interventions and strategic antimicrobial management.

Subjects

GENOTYPES; MICROBIAL sensitivity tests; PSEUDOMONAS aeruginosa; GENES; DRUG resistance in microorganisms; NOSOCOMIAL infections; ENTEROBACTERIACEAE

Publication

Journal of Pure & Applied Microbiology, 2024, Vol 18, Issue 2, p1004

ISSN

0973-7510

Publication type

Academic Journal

DOI

10.22207/JPAM.18.2.15