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- Title
The Complete Chloroplast Genome of Medinilla tapete-magicum (Melastomataceae) from Sulawesi, Indonesia.
- Authors
Priyadi, Arief; Kuswandi, Paramita Cahyaningrum; Yulianti, Evy; Pratiwi, Risha Amilia; Asih, Ni Putu Sri
- Abstract
In this study, the genome of an endemic Sulawesi's plant, Medinilla tapetemagicum was sequenced using Illumina NextSeq 500 and assembled the whole chloroplast genome. Results showed that the cpGenome is 155,602 bp in size with typical quadripartite structure of a large single copy (LSC) region (85,409 bp), a short single copy (SSC) region (16,629 bp), and a pair of inverted repeats (IRs) regions (26,782 bp). The cpGenome is composed of 132 genes, which consists of 87 protein coding genes, 37 tRNAs, and 8 rRNAs. The sliding window analyses showed that psbB-psbH and ndhF-rpl32 can potentially be used as markers. Microsatellite motifs of mononucleotide A and T dominated in the cpGenome. The phylogenetic trees from the concatenated 76 shared protein coding gene sequences showed the Medinilla clade was monophyletic and M. tapete-magicum is a sister species in the SE Asian clade which contain M. magnifica and M. speciosa.
- Subjects
INDONESIA; CHLOROPLASTS; MELASTOMATACEAE; MICROSATELLITE repeats; PHYLOGENY
- Publication
Journal of Tropical Biodiversity & Biotechnology, 2024, Vol 9, Issue 2, p1
- ISSN
2540-9573
- Publication type
Academic Journal
- DOI
10.22146/jtbb.87932