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- Title
A Simple Technique for Three-Dimensional Imaging and Segmentation of Brain Vasculature U sing Fast Free-of-Acrylamide Clearing Tissue in Murine.
- Authors
Khoradmehr, Arezoo; Mazaheri, Fahime; Anvari, Morteza; Tamadon, Amin
- Abstract
Fast Free-of-Acrylamide Clearing Tissue (FACT) is a recently developed protocol for the whole tissue three-dimensional (3D) imaging. The FACT protocol clears lipids using sodium dodecyl sulfate (SDS) to increase the penetration of light and reflection of fluorescent signals from the depth of cleared tissue. The aim of the present study was using FACT protocol in combination with imaging of auto-fluorescency of red blood cells in vessels to image the vasculature of a translucent mouse tissues.
- Publication
Cell journal, 2019, Vol 21, Issue 1, p49
- ISSN
2228-5806
- Publication type
Journal Article
- DOI
10.22074/cellj.2019.5684