Results
Title

تأثیر محیط کشت و هورمون های مختلف بر پرآوری و معماری ریشه گیاه دارویی زوفا در شرایط درون شیشهای)Hyssopus officinalis L.(

Authors

محمد سادات حسینی; ناصر عسکری

Abstract

Objective: The present study was performed to study the effects of MS, WPM, B5 culture media and benzylaminopurine (BAP) hormone on hyssop proliferation, and to assess the effects of different concentrations of IBA and IAA hormones on rooting characteristics of hyssop. Methods: The experiments were conducted as a factorial with a completely randomized design with three replications at the laboratory of tissue culture of the Faculty of Agriculture, University of Jiroft. To produce sterile seedlings, seeds were collected from mountainous regions of Kerman province and planted on the MS medium. The single-node explants were cultivated on MS, WPM, and B5 culture media containing the benzylaminopurine hormone with concentrations of 0, 1, and 2 mg/L. To induce rooting, the shoots were placed on the MS culture medium containing 0.5 and 1 mg/L of indole butyric acid and indole acetic acid. GiaRoots software was employed to analyze the architecture of the roots obtained. Results: Based on the research results, the highest proliferation coefficient was obtained in the MS culture medium with a concentration of 2 mg/L of benzylaminopurine hormone. In addition, the results of root architecture analysis showed that IBA hormone with a concentration of 1 mg/L demonstrated the most effective treatment for rooting induction. Conclusion: The adaptation test results demonstrated that 85% of the plants transferred to the greenhouse survived.

Subjects

INDOLEACETIC acid; BUTYRIC acid; TISSUE culture; BENZYLAMINOPURINE; PLANT micropropagation

Publication

Journal of Crops Improvement / Majallah-i Bih/zirā̒ī-i Kishāvarzī, 2024, Vol 26, Issue 2, p409

ISSN

2008-8337

Publication type

Academic Journal

DOI

10.22059/jci.2024.363422.2837