Evaluation of the Protective Effects of Doxycycline on Acetaminophen-Induced Hepatotoxicity in Mice.

Soltani, Shirin; Khodayar, Mohammad Javad; Yaghooti, Hamid; Salehcheh, Maryam; Mansouri, Esrafil; Zeidooni, Leila; Dehbashi, Fereshteh; Samimi, Azin

Acetaminophen (APAP) toxicity threatens human health due to increased mortality associated with its overdose. Doxycycline (DC) because of its properties such as antioxidant and anti-inflammatory can be a good therapeutic strategy to treat the acute toxicity induced by APAP. Male mice were divided into six groups in two periods of 3 h and 24 h as normal saline, APAP 400 mg/kg, DC 100 mg/kg and groups treated by 25, 50 and 100 mg/kg DC just before APAP, respectively. At the end of the 3 h and 24 h periods, the hepatic index, biochemical parameters including serum aspartate transaminase (AST) and alanine transaminase (ALT) activity and hepatic catalase activity, glutathione (GSH) and malondialdehyde (MDA) levels in liver and histopathological changes were evaluated. The results indicated that DC had no apparent effect on the hepatic index but significantly normalized the level of biochemical parameters and reduced APAP induced liver damage. Overall, it could be concluded that DC can inhibit or resolve harmful effects of APAP through antioxidant and anti-inflammatory properties. However, more studies are needed to understand exact mechanism of DC and its application for clinical use.

ACETAMINOPHEN; HEPATOTOXICOLOGY; DOXYCYCLINE; ALANINE aminotransferase; ASPARTATE aminotransferase; MICE

Iranian Journal of Pharmaceutical Research, 2019, Vol 18, Issue 2, p704

Academic Journal

10.22037/ijpr.2019.1100669