Title: Increased production of reactive oxygen species by the vacuolar-type (H<sup> </sup>)-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells.
Authors: Aya Yokomakura; JangJa Hong; Kazuo Ohuchi; Seong-Eun Oh; Jin-Yong Lee; Nariyasu Mano; Tsutomu Takahashi; Gi-Wook Hwang; Akira Naganuma
Abstract: Treatment of the mouse leukemic cell line RAW 264 with bafilomycin A1 or concanamycin A, inhibitors of vacuolar-type (H )-ATPases (V-ATPases), significantly increased the production of reactive oxygen species (ROS) and decreased cell viability. These effects were significantly suppressed by the presence of N-acetyl cysteine (NAC), an ROS scavenger. si-RNA mediated knockdown of the gene for the c subunit of the V0 domain of V-ATPase also resulted in an increase in ROS production and a decrease in cell viability. These results suggest that decreased cellular V-ATPase activity decreases cell viability by increasing ROS production in RAW 264 cells.
Subjects: REACTIVE oxygen species; ADENOSINE triphosphatase; ENZYME inhibitors; MACROLIDE antibiotics; SCAVENGER receptors (Biochemistry); CELL lines; CELL survival
Publication: Journal of Toxicological Sciences, 2012, Vol 37, Issue 5, p1045
ISSN: 0388-1350
Publication type: Academic Journal
DOI: 10.2131/jts.37.1045

Increased production of reactive oxygen species by the vacuolar-type (H<sup> </sup>)-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells.

Aya Yokomakura; JangJa Hong; Kazuo Ohuchi; Seong-Eun Oh; Jin-Yong Lee; Nariyasu Mano; Tsutomu Takahashi; Gi-Wook Hwang; Akira Naganuma