[Objectives] To optimize the fertilization technology of flue-cured tobacco Cuibi-1. [Methods] From 2015 to 2016, the experiment of spraying Saikun multi-trace element water-soluble fertilizer was carried out. [Results] 7 d and 14 d after transplanting tobacco seedlings, 3 750 mL/ha, 300 times diluted Sakun multi-trace element aqueous solution was sprayed, the growth period of Cuibi-1 in the field was extended by 3 -5 d. The incidence of mosaic disease at the rosette stage and granuviile wilt at the foot leaf harvesting and curing stage decreased by 4.50 - 20. 85 kg/ha, the yield increased by 67. 8 -68. 7 kg/ha, the proportion of high-quality tobacco increased by 1. 29 - 3. 01 percentage points, and the output value increased by 2 692. 5 -3 441 yuan/ha, the sensory smoking quality has improved. [ Conclusions] Increasing the application of multiple trace elements can increase the economic benefit and industrial use value of tobacco leaf production of Cuibi-1.