Establishment and Evaluation of a Mouse Model of Allergic Rhinitis.

Xiang QIN; Yuan PAN; Xiaoni PAN; Yanfeng MO; Shuwen LI; Tïngting XIE; Lixiang LU; Jiancheng LLANG; Shiyou ZHOU; Suoyi HUANG

[Objectives] To explore a new method for induction of allergic rhinitis in mice, and compare and evaluate it with common modeling methods. [Methods] 36 mice were randomly divided into the control group, blank group and experimental group, and there were 12 mice in each group. The mice in the control group were conventionally induced. That is, the mice were first injected intraperitoneally with the mixture composed of OVA 50 |xg, [A1(OH)3] 5 mg and 1ml of normal saline once every other day, and then since the 15*d, 20 p.L of 5% OVA solution was dropped into each nasal cavity once a day, which lasted for 7 d. The blank group was treated with the same amount of normal saline according to the control group, and received intraperitoneal injection and bilateral nasal drip respectively. In the experimental group, mice were first given intraperitoneal injection of the mixture composed of ovalbumin (OVA) 75 p,g, aluminum hydroxide gel [A1(OH)3] 8 mg and normal saline 1.5 mL for basic sensitization. On the 26* d, 20 p,L of 3% OVA solution was dropped into each nasal cavity once a day, which lasted for 10 d. The number of sneezes, the number of nose scratching, the amount of nasal discharge, and the activity of mice in each group were observed, and the behavior of allergic reaction was scored. Meanwhile, the number of eosinophils in the nasal discharge of mice and the IgE content in serum were measured. [Results] The score of nasal stimulation symptoms, the number of eosinophils and serum IgE level of mice in the control group and the experimental group were higher than those in the blank group (P < 0.05), and there was no statistical significance between the two groups in the three indicators (P >0.05). [Conclusions] The modeling method was more suitable for the development of allergic rhinitis patients' condition, and reduced the probability of death of mice due to modeling, and simplified the experimental operation.

ALLERGIC rhinitis; NASAL cavity; ALUMINUM hydroxide; ALLERGIES; LABORATORY mice; INTRAPERITONEAL injections

Medicinal Plant, 2024, Vol 15, Issue 4, p111

2152-3924

Academic Journal

10.19600/j.cnki.issn2152-3924.2024.04.025