Optimization of Preparation of Oregano Oil Microspheres by Box-Behnken Response Surface Methodology.

Fei HAN; Mengyao TU; Hui YANG; Hekun DUAN; Fuhao HU; Xinli LIANG; Yang GUAN; Wei XU

[Objectives] To optimize the formulation and preparation of oregano oil microspheres by Box-Behnken response surface methodology. [Methods] Chitosan was used as the carrier material to prepare oregano oil microspheres by emulsion crosslinking method. The encapsulation efficiency, drug loading and ZD30 were used as the evaluation indicators, and the comprehensive score (OD) obtained by "coefficient of variation-AHP comprehensive weighting method" was used as the final evaluation indicator. The formulation design and preparation process were optimized by single factor experiment and Box-Behnken response surface methodology, and the optimal process parameters were determined. [Results] The optimal formulation and preparation process parameters of oregano oil microspheres were as follows; the ratio of oregano oil to chitosan was 2: 1, the emulsifying speed of double emulsion was 200 r/min, the amount of emulsifier in the colostrum was 4%, and the volume of curing agent was 1. 0 mL. The average encapsulation efficiency was 45.33% ± 1.32%, the average drug loading was 30.59% ±2.45%, and the median diameter (ZD30) was 52.596 |r,m ±0.023%. [Conclusions] The encapsulation efficiency, drug loading and IDX of oregano oil chitosan microspheres prepared by emulsion crosslinking method met the requirements. The drug-loaded microsphere not only can be used as a preparation finished product for direct application, but also be used as a product intermediate to lay a foundation for the research and development of subsequent dosage forms.

RESPONSE surfaces (Statistics); MICROSPHERES; OREGANO; CHITOSAN; COLOSTRUM

Medicinal Plant, 2024, Vol 15, Issue 4, p40

2152-3924

Academic Journal

10.19600/j.cnki.issn2152-3924.2024.04.010