Effects of Exogenous Plant Hormones on Growth Status and Secondary Metabolism of Houttuynia cordata Thunb.

Xitao WANG; Kai YAN; Tanhua YU; Zhannan YANG; Shiqiong LUO

Objectives To improve the yield and secondary metabolite content of medicinal plants and to further develop and utilize the medicinal and other functions of medicinal plants. #Methods] We used the sterile tissue culture method with Houtuynia cordata Thunb. a the research object. Different concentrations of 1-naphthalene acetic acid (NAA), auxin (indole-3-acetic acid, IAA) and gibberellin acid (GA3) were added to the group culture medium of H. cordata to investigate the effects of exogenous plant hormones on plant height, root length, fresh weight, morphological characteristics, four phenolics and 20 volatile compounds. [Results] The results showed that the exogenous plant hormone of 3 mg/L GA3 significantly increased plant height by 79. 9% over the control; the exogenous plant hormone of 3 mg/L IAA significantly increased root length by 52. 6% over the control; and the exogenous plant hormone of 1 mg/L GA3 significantly increased fresh weight of single plant by 458.2% over the control. In the treatment group of 1 mg/LNAA, chlorogenic acid content was significantly increased by 52.6% com¬pared with the control; in the treatment group of 1 mg/L IAA, chlorogenic acid, rutin, isodendrin and quercetin content were significantly in¬creased by 109.1%, 100. 6%, 173. 8%, and 198. 7% compared with the control, respectively; in the treatment of 3 mg/L GA3, chlorogenic acid, rutin, isoquercitin, and quercitin content were significantly increased by 65. 3%, 104.9%, 139.0% and191.2% over the control. In addition, the content of volatile compounds was significantly higher in all H cordata treated with exogenous plant hormones of 2 mg/L NAA, 1 mg/L IAA, and 3 mg/L GA3; however, the content of volatile compounds was lower in aH of the treatments with 2 mg/L GA3. # Conclusions ] Different exogenous plant hormones have certain effects on the growth morphology and secondary metabolic content of H cordata, which provides theoretical basis and technical support for the development and utilization of medicinal plants.

PLANT hormones; CHLOROGENIC acid; GIBBERELLIC acid; SECONDARY metabolism; ACETIC acid

Medicinal Plant, 2024, Vol 15, Issue 1, p1

2152-3924

Academic Journal

10.19600/j.cnki.issn2152-3924.2024.01.001