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- Title
In-vivo Efficacy of Poultry and Fish Probiotics on Green Mussel, Perna viridis, Resistance against Vibrio alginolyticus.
- Authors
Laith, A. A.; Badr, S.; Ros-Amira, M. K.; Hassan, I. S.; Effendy, A. W. M.; Najiah, M.
- Abstract
The efficiency of using lactic acid bacteria derived from chicken to be used in fish to limit microbial infections in aquaculture was investigated. Gram-positive isolates were cultured on Man Rogosa Sharp agar (MRS). Identification of bacterial strains was done by analyzing nucleotide sequence of the 16S rRNA gene. Lactobacillus plantarum (98%) was isolated from Oreochromis nllotlcus and Enterococcus durans (96%) was isolated from Gallus gallus. The sequences were deposited in GenBank with the Accession No. of MF197402 and MF197403, respectively. Experimental mussels were divided into 3 groups; control group fed commercial feed only, Treatment 1 and Treatment 2 were fed with commercial feed supplemented with the candidate probiotics isolated from chicken and fish, respectively, for 21 days. P. viridis were immersed in V. alginolyticus 1.0 x 106 CFU/ml. L. plantarum significantly affected haemocyte count, lysozyme activity and mortality rate, whereas E. durans significantly affect lysozyme activity only.
- Subjects
LACTIC acid bacteria; PERNA; LACTOBACILLUS plantarum; PROBIOTICS; VIBRIO alginolyticus; NUCLEOTIDE sequence; MUSSELS; ENTEROCOCCAL infections
- Publication
Indian Journal of Animal Research, 2020, Vol 54, Issue 12, p1532
- ISSN
0367-6722
- Publication type
Academic Journal
- DOI
10.18805/ijar.B-1158