Results
Title

Incidence of bla<sub>IMP</sub> and bla<sub>VIM</sub> Genes among Carbapenemase Producing Escherichia coli in Lahore, Pakistan.

Authors

Khan, Shafqat Husnain; Jahan, Shah; Ahmad, Irshad; Rahman, Sadeeq ur; Rehman, Tayyab ur

Abstract

Antibiotic drug resistance in E. coli is a worldwide health problem. Particularly, increasingly reported carbapenem resistance due to carbapenemases is highly worrisome. Literature regarding incidence of class B metallo-β-lactamases (MBLs) - producing E. coli has not yet judiciously been addressed. In this study, we investigated the occurrence of carbapenemase- and MBLs-producers among a collection of 100 carbapenem resistant E. coli obtained from a tertiary hospital at Lahore Pakistan. All carbapenemase producers were further investigated to identify frequency of blaIMP and blaVIM encoding genes. Results of modified hodge test identified 81% carbapenemase producers, while combined disc diffusion test identified 28 isolates as MBL producers among them. Of all carbapenemase producers, 25 isolates (30.8%) of carbapenemase-producing E. coli were found to harbor bla-VIM gene, while 13 isolates (16.04%) were found to carry blaIMP gene. We report on high incidence rate of carbapenemase producers among carbapenem resistant E. coli isolates.

Subjects

LAHORE (Pakistan); PAKISTAN; CARBAPENEMASE; CARBAPENEMS; ESCHERICHIA coli; BETA lactamases; GENES; DRUG resistance; DIFFUSION

Publication

Pakistan Journal of Zoology, 2019, Vol 51, Issue 5, p1959

ISSN

0030-9923

Publication type

Academic Journal

DOI

10.17582/journal.pjz/2019.51.5.sc1