Weeds are exposed to light that induces different responses that increase or reduce growth and biomass. Light duration has an important role in plant growth development and allows plants to work properly. Therefore, the aim of this study was to determine the effect of light duration on the growth performance of Parthenium hysterophorus and Cannabis sativa. The results showed that under reduced light duration (2 hours), plant growth performance (i.e., biomass, plant height, leaf area, and number of branches) was all reduced. In addition, with increasing light duration (9 hours), both species grew faster and recorded their maximum biomass plant height, leaf area, and number of branches. Both weed species grown under reduced light duration could not reach maturity and complete their life cycles. P. hysterophorus has the potential to grow quickly and replace C. sativa under 9 hours of light duration, which is suitable for enhancing the medicinal value of C. sativa.