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- Title
Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughness.
- Authors
Yuchun Xie; Nai Rile; Xuewu Li; Haijun Li; Meng Zhao; Tianyu Che; Ting Cai; Zhihong Liu; Jinquan Li
- Abstract
The Inner Mongolia cashmere goat is famous for its bright white cashmere fibre. However, little attention is given to the excellent characteristics of this breed’s meat. We used label-free proteomics to analyse the total protein content in five different muscles, and 1 227 proteins were detected. Through sequential windowed acquisition of all theoretical fragment ions (SWATH), 16, 33, 49, 39, and 31 differentially expressed proteins were successfully detected in the five muscles. Protein–protein interaction network analysis of differentially expressed proteins revealed many strong interactions related to fatty acid beta oxidation and muscle development. Based on SWATH in five muscles, 25 differentially expressed proteins related to muscle development were detected, including seven muscle fibre structural proteins (ACTG2, ACTN4, TAGLN, MYL3, MYL1, MYL6B and MYH4). Finally, immunohistochemical analysis of MYL3 showed that the proportion of MYL3 may be a potential molecular marker for muscle toughness.
- Subjects
INNER Mongolia (China); GOAT meat; CYTOSKELETAL proteins; FATTY acid oxidation; MUSCLE growth; CASHMERE; FOOD labeling
- Publication
Czech Journal of Animal Science, 2022, Vol 67, Issue 4, p137
- ISSN
1212-1819
- Publication type
Academic Journal
- DOI
10.17221/61/2021-CJAS