广叶绣球菌多糖对铅暴露小鼠海马损伤缓解作用.

吴 蕊; 冯 瑞; 牛旭凯; 许梦燕; 韩笑月; 云少君; 冯翠萍; 曹谨玲

Kunming mice were randomly divided into blank control (NC) group, lead exposure (PB) group, Sparassis latifolia polysaccharide (SLP) group, and lead exposure (Co-PB) group co-housed with SLP treatment (Co-SLP) group. Except NC group, mice in the rest groups were given 1 g·L−1 lead acetate solution as drinking water, and mice in SLP and Co-SLP groups were also administered 400 mg·kg−1 SLP by gavage. After 8 weeks, mice in different groups were measured for lead content in hippocampus by graphite furnace atomic absorption spectrometry, observed for hippocampal tissue structure by hematoxylin-eosin staining, determined for contents of malondialdehyde (MDA) and glutathione (GSH), and determined for activities of total superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in hippocampus. Real-time quantitative PCR was used to detect relative expression of inflammation-related genes IL-4, IL-6, IL-10, IL-1β, TNF-α, TGF-β, and autophagy related genes Beclin-1, LC3-I, LC3-II, Lamp2, P62 and Atg5 in hippocampus. The results showed that the hippocampal lead contents of SLP, Co-SLP and Co-PB were significantly lower than that of PB. There were significant increases of CAT activity and GPx activity in SLP mice and Co-SLP mice compared with PB mice. The SOD activity and GSH content of SLP, Co-SLP and Co-PB groups were also increased significantly. On the other hand, the contents of MDA in SLP, CoSLP and Co-PB groups decreased significantly compared with that in PB group. Compared with PB mice, mice treated with SLP showed significantly decreased expression of IL-1β, IL-6 TNF-α, LC3II, Lamp2, Beclin1, LC3-I and Atg5, and significantly increased expression of IL-4, TGF-β and IL-10. Compared with PB mice, Co-SLP mice showed significantly decreased expression of IL-6, TNF-α, LC3 II, Lamp2, Beclin1, LC3-I and Atg5, and significantly increased expression of IL-4, TGF-β, IL-10 and P62. Compared with PB mice, Co-PB mice showed significantly decreased expression of TNF-α, LC3-I and Atg5, and significantly increased expression of TGF-β and IL-10. The results provided a reference for ameliorating lead injury by S. latifolia polysaccharide

FURNACE atomic absorption spectroscopy; LEAD exposure; LEAD; POLYSACCHARIDES; SUPEROXIDE dismutase; MALONDIALDEHYDE

Acta Edulis Fungi, 2024, Vol 31, Issue 6, p50

1005-9873

Academic Journal

10.16488/j.cnki.1005-9873.2024.06.006