Screening and Virulence Characterization of Vibrio parahaemolyticus with High Production of AI-2.

YUAN Shuo; WANG Yanbo; LIU Shuilin; CUI Fangchao; TAN Xiqian; LÜ Xinran; LI Jianrong; LI Xuepeng; DONG Hao

Vibrio parahaemolyticus is a common foodborne pathogen whose motility, biofilm formation and virulence factor production are regulated by AI-2/LuxS quorum sensing system. In this study, V. parahaemolyticus with high yield of AI- 2 signaling molecules was selected from nine strains Vibrio, and effects of exogenous AI -2 signaling molecules on biofilm, virulence factors and motility of V. parahaemolyticus were analyzed. The results showed that a strain of V. parahaemolyticus VP-4 with high production of AI-2 signaling molecules was obtained, and the promotion rates of biofilm, gelatinase, lecithin enzyme, amylase, motility, and hemolysis by adding exogenous AI-2 signaling molecules with different volume fractions (1%-7%) were 33.02%-67.87%, 1.67%-7.26%, 3.25%-48.15%, 1.21%-12.17%, 4.92%-35.98%, 4.15%-50.60%, and 2.06%-15.23%, respectively. Meanwhile, scanning electron microscope observation showed that the addition of exogenous AI-2 signaling molecules caused biofilm of V. parahaemolyticus to accumulate, increased in thickness and compact structure. The above results indicated that AI-2 signaling molecules could promote the formation of V. parahaemolyticus biofilm and the release of virulence factors, as well as enhance its motility. This study could provide a novel target for controlling the pathogenicity of Vibrio parahaemolyticus in seafood.

VIBRIO parahaemolyticus; SCANNING electron microscopes; QUORUM sensing; FACTORS of production; FOOD pathogens

Journal of Chinese Institute of Food Science & Technology / Zhongguo Shipin Xuebao, 2025, Vol 25, Issue 2, p115

1009-7848

Academic Journal

10.16429/j.1009-7848.2025.02.010