Results
Title

The Difference of Biosynthesis of Flavonoids in Goji Berry from Different Regions Based on Proteomic Analysis.

Authors

Sun Xiazhi; Ma Ruixue; Yang Chao; Fan Yanli

Abstract

Objective: To analyze the differences of goji flavonoids from different origins from the perspective of proteomics. Methods: The protein differences of goji berries from different origins were explored by tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology in Ningxia, Qinghai and Gansu. Results: A total of 716 differential expressed proteins (DEPs), and a total of four metabolic pathways associated with flavonoids were found in the KEGG pathway, and the phenylpropane biosynthesis pathway was significantly enriched in all three comparison groups (P < 0.05), indicating that the phenylpropane biosynthesis pathway might make a greater contribution to the synthesis of goji flavonoids. Further screening of 24 key differentially expressed proteins related to flavonoid synthesis, it was found that phenylalanine ammonia-lyase (PAL), cinnamic acid 4-hydroxylase (C4H), chalcone isomerase (CHI), chalcone synthase (CHS), etc. might be important DEPs for the biosynthesis of flavonoids in Ningxia, Qinghai and Gansu. Conclusion: The differential proteins related to the biosynthesis of goji flavonoids were identified from the protein level, and the anabolic pathway of goji flavonoids was preliminarily speculated, which laid a foundation for exploring the differences in the biosynthesis of goji flavonoids in different origins.

Subjects

CHALCONE synthase; CINNAMIC acid; PROTEIN synthesis; BIOSYNTHESIS; FLAVONOIDS; LIQUID chromatography-mass spectrometry; TANDEM mass spectrometry

Publication

Journal of Chinese Institute of Food Science & Technology / Zhongguo Shipin Xuebao, 2024, Vol 24, Issue 11, p348

ISSN

1009-7848

Publication type

Academic Journal

DOI

10.16429/j.1009-7848.2024.11.032