Results
Title

Molecular Phylogenetics of Physa acuta (Pulmonata: Basommatophora): an Invasive species in Central Punjab Pakistan.

Authors

Ansari, B.; Altafa, J.; Ramzan, A.; Ahmed, Z.; Khalil, S.; Qamar, S. U. R.; Awan, S. A.; Jehangir, K.; Khalid, R.; Aziz, S.; Sultana, T.; Sultana, S.; Alsamadany, H.; Alshamrani, R.; Awan, F. S.

Abstract

Physids belong to Class Gastropoda; belong to Phylum Mollusca and being bioindicators, intermediate hosts of parasites and pests hold a key position in the ecosystem. There are three species of Genus Physa i.e. P. fontinalis, Physa acuta and P. gyrina water bodies of Central Punjab and were characterized on the basis of molecular markers High level of genetic diversity was revealed by polymorphic RAPD, however SSR markers were not amplified. The multivariate analysis revealed polymorphism ranging from 9.09 percent to 50 percent among the three Physid species. Total number of 79 loci were observed for the three species under study and 24 loci were observed to be polymorphic. These RAPD fragment(s) can be developed into co dominant markers (SCAR) by cloning and can be further sequenced for the development of the Physa species specific markers to identify the introduced and native species in Pakistan.

Subjects

PAKISTAN; PUNJAB; MOLECULAR phylogeny; PULMONATA; INTRODUCED species; GENETIC variation; BODIES of water

Publication

Brazilian Journal of Biology, 2023, Vol 83, p1

ISSN

1519-6984

Publication type

Academic Journal

DOI

10.1590/1519-6984.246984