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Title

Myosin X Interaction with KIF13B, a Crucial Pathway for Netrin-1-Induced Axonal Development.

Authors

Hua-Li Yu; Yun Peng; Yang Zhao; Yong-Sheng Lan; Bo Wang; Lu Zhao; Dong Sun; Jin-Xiu Pan; Zhao-Qi Dong; Lin Mei; Yu-Qiang Ding; Xiao-Juan Zhu; Wen-Cheng Xiong

Abstract

Myosin X (Myo X) transports cargos to the tips of filopodia for cell adhesion, migration, and neuronal axon guidance. Deleted in Colorectal Cancer (DCC) is one of the Myo X cargos that is essential for Netrin-1-regulated axon pathfinding. The function of Myo X in axon development in vivo and the underlying mechanisms remain elusive. Here, we provide evidence for the role of Myo X in Netrin-1-DCC-regulated axon development in developing mouse neocortex. The knockout (KO) or knockdown (KD) of Myo X in cortical neurons of embryonic mouse brain impairs axon initiation and contralateral branching/targeting. Similar axon deficits are detected in Netrin-1-KO or DCC-KD cortical neurons. Further proteomic analysis of Myo X binding proteins identifies KIF13B (a kinesin family motor protein). The Myo X interaction with KIF13B is induced by Netrin-1. Netrin-1 promotes anterograde transportation of Myo X into axons in a KIF13B-dependent manner. KIF13B-KD cortical neurons exhibit similar axon deficits. Together, these results reveal Myo X-KIF13B as a critical pathway for Netrin-1- promoted axon initiation and branching/targeting.

Subjects

MYOSIN; MOLECULAR motor proteins; CARRIER proteins; CELL adhesion; AXONS

Publication

Journal of Neuroscience, 2020, Vol 40, Issue 48, p9169

ISSN

0270-6474

Publication type

Academic Journal

DOI

10.1523/JNEUROSCI.0929-20.2020