Chronic Resveratrol Treatment Protects Pancreatic Islets against Oxidative Stress in db/db Mice.

Young-Eun Lee; Ji-Won Kim; Eun-Mi Lee; Yu-Bae Ahn; Ki-Ho Song; Kun-Ho Yoon; Hyung-Wook Kim; Cheol-Whee Park; Guolian Li; Zhenqi Liu; Seung-Hyun Ko

Resveratrol (RSV) has anti-inflammatory and anti-oxidant actions which may contribute to its cardiovascular protective effects. We examined whether RSV has any beneficial effects on pancreatic islets in db/db mice, an animal model of type 2 diabetes. The db/db and db/dm mice (non-diabetic control) were treated with (db-RSV) or without RSV (db-control) (20 mg/kg daily) for 12 weeks. After performing an intraperitoneal glucose tolerance test and insulin tolerance test, mice were sacrificed, the pancreas was weighed, pancreatic β-cell mass was quantified by point count method, and the amount of islet fibrosis was determined. 8-Hydroxydeoxyguanosine (8-OHdG), an oxidative stress marker, was determined in 24 h urine and pancreatic islets. RSV treatment significantly improved glucose tolerance at 2 hrs in db/db mice (P = 0.036), but not in db/dm mice (P = 0.623). This was associated with a significant increase in both pancreas weight (P = 0.011) and β-cell mass (P = 0.016). Islet fibrosis was much less in RSV-treated mice (P = 0.048). RSV treatment also decreased urinary 8-OHdG levels (P = 0.03) and the percentage of islet nuclei that were positive for 8-OHdG immunostaining (P = 0.019). We conclude that RSV treatment improves glucose tolerance, attenuates β-cell loss, and reduces oxidative stress in type 2 diabetes. These findings suggest that RSV may have a therapeutic implication in the prevention and management of diabetes

OXIDATIVE stress; TREATMENT of diabetes; LABORATORY mice; ISLANDS of Langerhans; INSULIN resistance; PANCREATIC beta cells

PLoS ONE, 2012, Vol 7, Issue 11, p1

1932-6203

Academic Journal

10.1371/journal.pone.0050412