益生菌固态发酵独活茎叶工艺的优化.

白长胜; 刘雪松; 孟维珊; 钟鹏; 史同瑞

The purpose of this experiment was to optimize the process of probiotics solid-state fermentation on stems and leaves of Angelica pubescens Radix, and to increase the content of osthole in stems and leaves of Angelica pubescens Radix. In this experiment, Bacillus amyloliquefaciens strain SSYB was used to ferment the stems and leaves of Angelica pubescens Radix, and on the basis of single factor test, the solid-state fermentation process was optimized by orthogonal experimental design, and the content of osthole in stems and leaves of Angelica pubescens Radix before and after the fermentation process was determined by high performance liquid chromatography. The results showed that the optimized fermentation process of stems and leaves of Angelica pubescens Radix was fermentation time of 72 h, fermentation temperature of 37 ℃, inoculation amount of 5%, and water content 65%. Under these conditions, the content of osthole in stems and leaves of Angelica pubescens Radix was 1.81 mg/g, which was significantly higher than that before fermentation by 45.97% (P<0.01). The results show that the content of osthole in stems and leaves of Angelica pubescens Radix is increased by optimizing the solid-state fermentation process of Bacillus amyloliquefaciens SSYB.

HIGH performance liquid chromatography; BACILLUS amyloliquefaciens; FERMENTATION; SOLID-state fermentation; EXPERIMENTAL design; PROBIOTICS

Feed Research, 2024, Vol 47, Issue 15, p62

1002-2813

Academic Journal

10.13557/j.cnki.issn1002-2813.2024.15.012