Objective: To investigate the effect of different incision phacoemulsification combined with trabeculectomy on vision, corneal endothelial cells and quality of life in cataract patients with glaucoma. Methods: The clinical data of 150 patients with cataract and glaucoma who were admitted to our hospital were analyzed retrospectively from April 2018 to July 2019. They were divided into group A (n=75, single incision phacoemulsification combined with trabeculectomy) and group B (n=75, double incision phacoemulsification combined with trabeculectomy) by different operation methods. The intraocular pressure, naked vision, best corrected vision, corneal endothelial cells, quality of life and complications were compared between the two groups. Results: The scores of health survey form (SF-36) in the two groups were higher than those in group A (P < 0.05). The intraocular pressure in group B was lower than that in group A (P < 0.05), and the uncorrected visual acuity and the best corrected visual acuity in group B were higher than that in group A (P < 0.05). The area of corneal endothelial cells in group B was smaller than that in group A (P < 0.05), and the density of corneal endothelial cells in group B was lower than that in group A (P < 0.05). The total incidence of postoperative complications in group B was lower than that in group A (P< 0.05). Conclusion: Compared with single incision phacoemulsification combined with trabeculectomy, double incision phacoemulsification combined with trabeculectomy is more effective in improving intraocular pressure, naked eye vision, best corrected vision, corneal endothelial cells, quality of life and reducing complications.