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- Title
Effects of Xinwei granules on the METTL16/CyclinD1 pathway in gastric cancer cell proliferation, apoptosis, and cell cycle.
- Authors
WU Lili; ZHANG Ran; LIU Xinyao; ZHANG Yang; WANG Jingbin
- Abstract
Objective: To investigate the effects of Xinwei granules on the METTL16/CyclinD1 pathway and proliferation, apoptosis, and cell cycle of gastric cancer cells, and to elucidate the molecular mechanisms underlying its therapeutic potential in gastric cancer. Methods: Cell viability of the MKN-45 gastric cancer cell line was assessed using the CCK-8 assay following treatment with various concentrations of gastric-derived granules. Flow cytometry was employed to analyze apoptosis and cell cycle distribution. Western blot and RT-qPCR analyses were used to measure the expression of key molecules in the METTL16/CyclinD1 pathway. Spot hybridization was performed to detect m6A methylation modifications of CyclinD1. Results: Compared to the control group, gastric-derived granules significantly increased apoptosis in MKN-45 cells, elevated the proportion of cells in the G1 phase, reduced the proportion in the S phase, and downregulated the expression of CyclinD1, CDK4, E2F1, and METTL16 proteins as well as the p-Rb/Rb ratio (P<0.01) . Additionally, gastric-derived granules significantly decreased the mRNA expression levels of METTL16 and CyclinD1, and inhibited CyclinD1 m6A methylation modifications (P<0.01). Conclusion: Xinwei granules exert their effects by inhibiting METTL16-mediated m6A methylation of CyclinD1, thereby blocking the transition from the G1 phase to the S phase, suppressing cell proliferation, and promoting apoptosis.
- Subjects
CANCER cell proliferation; CELL cycle; STOMACH cancer; WESTERN immunoblotting; PRECANCEROUS conditions
- Publication
Journal of Hainan Medical University, 2024, Vol 30, Issue 24, p1869
- ISSN
1007-1237
- Publication type
Academic Journal
- DOI
10.13210/j.cnki.jhmu.20241022.001