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- Title
Determination of Tannins Content and Antibacteria Activity Test of Ethanol Extract of Sirih Merah (Piper Crocatum Ruiz & pav.) Leaf from North Sumatera Province Indonesia.
- Authors
GURNING, KASTA; SITUMORANG, RISANTI FEBRINE ROPITA; SINAGA, EFEREDDY TEMEN; SINGARIMBUN, NURBAITI; SINAGA, SUHARNI PINTAMAS; SILABAN, SARONOM
- Abstract
Sirih merah (Piper crocatum Ruiz & Pav.) leaves has traditionally been used as a medicinal plant. The content of secondary metabolites contained is assessed to show pharmacological activity. The secondary metabolites in question include tannins, alkaloids, flavonoids, steroids/triterpenoids and saponins. This study aimed to determine the total tannin content and the potential antibacterial activity of the ethanol extract of P. crocatum leaves. Extraction process by maceration using ethanol, measurement of total tannin content by colorimetric method using UV-Vis spectrophotometry at a maximum wavelength of 745 nm and determination of antibacterial activity by disc diffusion. The results of this study showed that the ethanolic extract of P. crocatum leaves contained about 0.118±0.003 mg TAE/g of dry ethanolic extract. The results of activity testing against Escherichia coli and Staphylococcus aureus showed activity as antibacterial.
- Subjects
INDONESIA; TANNINS; METABOLITES; STAPHYLOCOCCUS aureus; ETHANOL; ANTIBACTERIAL agents; ESCHERICHIA coli
- Publication
Oriental Journal of Chemistry, 2022, Vol 38, Issue 4, p967
- ISSN
0970-020X
- Publication type
Academic Journal
- DOI
10.13005/ojc/380418