Results
Title

Determination of Tannins Content and Antibacteria Activity Test of Ethanol Extract of Sirih Merah (Piper Crocatum Ruiz & pav.) Leaf from North Sumatera Province Indonesia.

Authors

GURNING, KASTA; SITUMORANG, RISANTI FEBRINE ROPITA; SINAGA, EFEREDDY TEMEN; SINGARIMBUN, NURBAITI; SINAGA, SUHARNI PINTAMAS; SILABAN, SARONOM

Abstract

Sirih merah (Piper crocatum Ruiz & Pav.) leaves has traditionally been used as a medicinal plant. The content of secondary metabolites contained is assessed to show pharmacological activity. The secondary metabolites in question include tannins, alkaloids, flavonoids, steroids/triterpenoids and saponins. This study aimed to determine the total tannin content and the potential antibacterial activity of the ethanol extract of P. crocatum leaves. Extraction process by maceration using ethanol, measurement of total tannin content by colorimetric method using UV-Vis spectrophotometry at a maximum wavelength of 745 nm and determination of antibacterial activity by disc diffusion. The results of this study showed that the ethanolic extract of P. crocatum leaves contained about 0.118±0.003 mg TAE/g of dry ethanolic extract. The results of activity testing against Escherichia coli and Staphylococcus aureus showed activity as antibacterial.

Subjects

INDONESIA; TANNINS; METABOLITES; STAPHYLOCOCCUS aureus; ETHANOL; ANTIBACTERIAL agents; ESCHERICHIA coli

Publication

Oriental Journal of Chemistry, 2022, Vol 38, Issue 4, p967

ISSN

0970-020X

Publication type

Academic Journal

DOI

10.13005/ojc/380418