Fungal Trichoderma harzianum and Pleurotus ostreatus contained in the pro-compost shows the ability to produce enzymes. which can overhaul the structure of polyurethane so that it breaks down easily in the environment. Polyurethane degradation was carried out by using the blackout method through the compost pro medium. Some characterization techniques carried out include techniques to determine mass loss and polyurethane degradability. Analysis of functional groups with Fourier Transform Infra-Red (FTIR), and surface observation with the Scanning Electron Microscopy technique (SEM). Based on the results of quantitative analysis during biodegradation, there is a mass loss of 87.5% in polyurethanes synthesized from PEG, MDI, KBH starch and degradability 0.1987 g/day. FTIR test showed that before degradation there was a urethane functional group C = O and an N-H functional group after the degradation of urethane functional group C = O and the N-H group is absent in the samples.