Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry.

Hani, Saleem Bani; Bayachou, Mekki

Objective To investigate the role of water-soluble extract of Salvia fruticosa (Greek sage) ( S. fruticosa ) leaves in reducing both intrinsic cellular and H 2 O 2 -induced DNA oxidation in cultured human embryonic kidney 293 cells. S. fruticosa , native to the Eastern-Mediterranean basin, is widely used as a medicinal herb for treatment of various diseases. Methods Dried leaves of S. fruticosa were extracted in phosphate buffer saline and purified using both vacuum and high pressure filtrations. Each mL of the preparation contained (7.1×1.0) mg of extract. HEK-293 cells were incubated in one set with S. fruticosa extract in the presence of 0.1 mmol/L H 2 O 2 , and in the other set with the addition of the extract alone. The DNA oxidation was measured using fluorescence upon fluorescein isothiocyanate derivatization of 8-oxoguanine moieties. The fluorescence was measured using flow cytometry technique. Results Cells incubated 3 h with 150 μL extract and exposed to 0.1 mmol/L H 2 O 2 showed lower intensity of fluorescence, and thus lower DNA oxidation. Moreover, cells incubated 3 h with 100 μL of the extract showed lower intensity of fluorescence, and thus lower intrinsic cellular DNA oxidation compared to control (without S. fruticosa ). Conclusions The results from this study suggest that the water-soluble extract of S. fruticosa leaves protects against both H 2 O 2 -induced and intrinsic cellular DNA oxidation in human embryonic kidney 293 cells.

MEDITERRANEAN Region; HERBAL medicine; HYDROGEN peroxide; OXIDATION of DNA; FLOW cytometry; COMPOSITION of leaves; SALVIA

Asian Pacific Journal of Tropical Biomedicine, 2014, Vol 4, Issue 5, p399

2221-1691

Academic Journal

10.12980/APJTB.4.2014C1270