Title: Construction of Chimeric Glucansucrases for Analyzing Substrate-binding Regions That Affect the Structure of Glucan Products.
Authors: Funane, Kazumi; Ishu, Tadashi; Terasawa, Kazue; Yamamoto, Tomoko; Kobayashi, Mikihiko
Abstract: Constructs six chimeric enzymes from two recombinant glucansucrases, dextransucrase S (DSRS) and dextransucrase 5 (DSRT5), by exchanging three substrate-binding regions in the N-terminal catalytic region. Effects of the regions on enzyme activity and the structures of the glucan products; Construction of chimeric DS genes; Preparation of glucansucrase chimeric enzymes.
Subjects: ENZYMES; BINDING sites; CATALYSTS; GLUCANS; GENES; PROTEINS
Publication: Bioscience, Biotechnology & Biochemistry, 2004, Vol 68, Issue 9, p1912
ISSN: 0916-8451
Publication type: Academic Journal
DOI: 10.1271/bbb.68.1912

Construction of Chimeric Glucansucrases for Analyzing Substrate-binding Regions That Affect the Structure of Glucan Products.

Funane, Kazumi; Ishu, Tadashi; Terasawa, Kazue; Yamamoto, Tomoko; Kobayashi, Mikihiko