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Title

Identification of Catalytic Amino Acids of Cyclodextran Glucanotransferase from Bacillus circulans T-3040.

Authors

Yamamoto, Tomoko; Terasawa, Kazue; Young-Min Kim; Kimura, Atsuo; Kitamura, Yoshiaki; Kobayashi, Mikihiko; Funane, Kazumi

Abstract

The article presents a report identifying catalytic amino acids of cyclodextran glucanotransferase (CITase) from Bacillus circulans T-3040. CITase was modified chemically from the T-3040 strain of Baciullus circulans. The results show that Asp145 and Asp270 are important in the enzymatic reaction of T-3040 CITase.

Subjects

AMINO acids; ORGANIC acids; ENZYMES; CATALYSTS; CHEMICAL processes

Publication

Bioscience, Biotechnology & Biochemistry, 2006, Vol 70, Issue 8, p1947

ISSN

0916-8451

Publication type

Academic Journal

DOI

10.1271/bbb.60105

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