Title: Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana.
Authors: Gao, Yongshun; Badejo, Adebanjo Ayobamidele; Shibata, Hitoshi; Sawa, Yoshihiro; Maruta, Takanori; Shigeoka, Shigeru; Page, Mike; Smirnoff, Nicholas; Ishikawa, Takahiro
Abstract: The article presents a study which investigates the expression levels of both VTC2 and VTC5 gene encoding GDP-L-galactose phosphorylase, an enzyme involved in Ascorbate (AsA) Biosynthesis in Arabidopsis thaliana. The study used luciferase activity assay, RNA isolation, and bipyridyl method using microtitier plate reader. Results show that the transcript level of VTC2 and VTC5 have increased in parallel with AsA accumulation and VTC5 compensated on induced VTC2 transcription levels.
Subjects: GENE expression; ARABIDOPSIS thaliana; GALACTOSE; PHOSPHORYLASES; LUCIFERASES; BIOSYNTHESIS
Publication: Bioscience, Biotechnology & Biochemistry, 2011, Vol 75, Issue 9, p1783
ISSN: 0916-8451
Publication type: Academic Journal
DOI: 10.1271/bbb.110320

Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana.

Gao, Yongshun; Badejo, Adebanjo Ayobamidele; Shibata, Hitoshi; Sawa, Yoshihiro; Maruta, Takanori; Shigeoka, Shigeru; Page, Mike; Smirnoff, Nicholas; Ishikawa, Takahiro