Results
Title

Construction of a chromosome-assigned, sequence-tagged linkage map for the radish, Raphanus sativus L. and QTL analysis of morphological traits.

Authors

Tomoko Hashida; Ryoichi Nakatsuji; Budahn, Holger; Schrader, Otto; Peterka, Herbert; Tatsuhito Fujimura; Nakao Kubo; Masashi Hirai

Abstract

The radish displays great morphological variation but the genetic factors underlying this variability are mostly unknown. To identify quantitative trait loci (QTLs) controlling radish morphological traits, we cultivated 94 F4 and F5 recombinant inbred lines derived from a cross between the rat-tail radish and the Japanese radish cultivar 'Harufiiku' inbred lines. Eight morphological traits (ovule and seed numbers per silique, plant shape, pubescence and root formation) were measured for investigation. We constructed a map composed of 322 markers with a total length of 673.6 cM. The linkage groups were assigned to the radish chromosomes using disomic rape-radish chromosome-addition lines. On the map, eight and 10 QTLs were identified in 2008 and 2009, respectively. The chromosome-linkage group correspondence, the sequence-specific markers and the QTLs detected here will provide useful information for further genetic studies and for selection during radish breeding programs.

Subjects

RADISHES; NUCLEOTIDE sequence; CHROMOSOMES; GENE mapping; QUANTITATIVE research; LOCUS (Genetics)

Publication

Breeding Science, 2013, Vol 63, Issue 2, p218

ISSN

1344-7610

Publication type

Academic Journal

DOI

10.1270/jsbbs.63.218