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Title

抗弧菌光合细菌的分离鉴定及对氨氮和亚硝态氮的降解特性.

Authors

王雨婷; 周荣翔; 李霁虹; 张 瑶; 周婷婷; 陈文才; 彭 云2,; 汤曼利; 马桂珍; 许建和

Abstract

In this study, photosynthetic bacteria (PSB) were isolated and purified from marine environmental samples from different areas by double-layer plate coating method and scribing method. Vibrio parahaemolyticus, V. vulnificus and V. anguillarum were used as control. The inhibition of marine photosynthetic bacteria was determined by Oxford cup method, and the degradation of nitrite nitrogen (NO2−-N) and ammonia nitrogen (NH4+-N) by different strains was determined by naphthalene ethylenediamine hydrochloride spectrophotometry and indophenol blue spectrophotometry. The results show that three strains of photosynthetic bacteria were isolated from 30 sea water and mud samples, and the P-3 strain isolated from the seawater samples of Cheniushan Island in Lianyungang had strong inhibitory effect on three kinds of Vibrio, especially for V. anguillarum, with the inhibition zone diameter of 5.3 mm. The results show that all the three photosynthetic bacteria have certain ability to degrade NO2−-N and NH4+-N, and P-3 strain had the strongest ability. The degradation rates of P-3 strain were 89.68% and 94.98% respectively, when being cultured in the medium containing 50 mg·L−1 NH4+-N and NO2−-N for 4 d. P-3 strain was identified as Rhodopseudomonas palustris by morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis.

Subjects

PHOTOSYNTHETIC bacteria; VIBRIO parahaemolyticus; RHODOPSEUDOMONAS palustris; MARINE bacteria; SEAWATER

Publication

South China Fisheries Science, 2021, Vol 17, Issue 5, p26

ISSN

2095-0780

Publication type

Academic Journal

DOI

10.12131/20210016

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