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Title

副溶血弧菌养殖对虾分离株耐药性及耐药基因分析.

Authors

魏文娟; 赵 姝; 王 元; 周俊芳; 李新苍; 房文红

Abstract

We used agar dilution method to detect the resistance of 36 strains of Vibrio parahaemolyticus to 16 drugs. And used PCR amplication and DNA sequencing to detect the antimicrobial resistance to quinolones (qnrA, qnrB, qnrS, qnrVC), phenicols (cat, optrA, floR, cfr), tetracyclines (tetA, tetB, tetM), sulfonamides (sul1, sul2, sul3), aminoglycosides (strA, strB, aadA, aacA), rifampicin (arr), β-lactams (carB) and macrolides (erm). The results indicate that the isolates exhibited high resistance to ampicillin (88.9%) and sulfamethoxazole (SMZ, 66.7%), all susceptible to neomycin sulfate, gentamicin, ceftriaxone and meropenem. In general, multi-drug resistance (MDR) was highly prevalent (61.1%), and one isolate was resistant to six antimicrobials. Furthermore, 72.2% and 58.3% of the isolates were primarily mediated by qnrVC and strB, respectively; and the macrolides and rifamycin resistant genes were not detected in all the isolates. Obvious mismatch was found between the antimicrobial resistance phenotypes and genotypes, revealing the complexity of resistance to V. parahaemolyticus.

Subjects

DRUG resistance in microorganisms; TETRACYCLINES; VIBRIO parahaemolyticus; MULTIDRUG resistance; GENTAMICIN

Publication

South China Fisheries Science, 2020, Vol 16, Issue 1, p9

ISSN

2095-0780

Publication type

Academic Journal

DOI

10.12131/20190165