Dynamic monitoring of platelet activation and its role in post-dissection inflammation in a canine model of acute type A aortic dissection.

Chaoyi Qin; Hongwei Zhang; Jun Gu; Zhenghua Xiao; Qin Yang; Wei Meng; Qin, Chaoyi; Zhang, Hongwei; Gu, Jun; Xiao, Zhenghua; Yang, Qin; Meng, Wei

Background: To confirm the activation of platelets (PLT) and explore the role of activated PLT in post-dissection inflammation.Method: An acute type A aortic dissection (AAD) canine model was established. Mean platelet volume/platelet count (MPV/PTC), platelet size distribution width (PDW), and inflammatory cytokines (tumor necrosis factor-α [TNF-α] and interleukin-6 [IL-6]) were measured between anesthetization and thoracotomy (T1), at the end of the operation (T2), and at 2 h (T3), 4 h (T4), and 6 h (T5) after the operation. Bivariate analysis was used to determine the correlations between the peak MPV/PTC, PDW, and inflammatory cytokines at T4.Result: An AAD canine model was successfully established. Both MPV/PTC and PDW values were significantly higher at T3-T5 than at T1 (P Conclusion: Activated PLT were identified after AAD, and played a critical role in the initiation of post-dissection inflammation.

BLOOD platelet activation; DISSECTION; CANIDAE; LABORATORY dogs; AORTIC dissection; INFLAMMATION; ANIMAL experimentation; BIOLOGICAL models; COMPARATIVE studies; DOGS; INTERLEUKINS; RESEARCH methodology; MEDICAL cooperation; RESEARCH; TUMOR necrosis factors; EVALUATION research; THORACIC aneurysms; PLATELET count; MEAN platelet volume; DISSECTING aneurysms

Journal of Cardiothoracic Surgery, 2016, Vol 11, p1

1749-8090

Academic Journal

10.1186/s13019-016-0472-5