Chamomile decoction extract inhibits human neutrophils ROS production and attenuates alcohol-induced haematological parameters changes and erythrocytes oxidative stress in rat.

Jabri, Mohamed-Amine; Sani, Mamane; Rtibi, Kais; Marzouki, Lamjed; El-Benna, Jamel; Sakly, Mohsen; Sebai, Hichem

Background: The aim of this study was to evaluate the protective effects of subacute pre-treatment with chamomile (Matricaria recutita L.) decoction extract (CDE) against stimulated neutrophils ROS production as well as ethanol (EtOH)-induced haematological changes and erythrocytes oxidative stress in rat. Methods: Neutrophils were isolated and ROS generation was measured by luminol-amplified chemiluminescence. Superoxide anion generation was detected by the cytochrome c reduction assay. Adult male wistar rats were used and divided into six groups of ten each: control, EtOH, EtOH various doses of CDE (25, 50, and 100 mg/kg, b.w.), and EtOH ascorbic acid (AA). Animals were pre-treated with CDE extract during 10 days. Results: We found that CDE inhibited (P ≤ 0.0003) luminol-amplified chemiluminescence of resting neutrophils and N-formyl methionylleucyl-phenylalanine (fMLF) or phorbolmyristate acetate (PMA) stimulated neutrophils, in a dose-dependent manner. CDE had no effect on superoxide anion, but it inhibited (P ≤ 0.0004) H2O2 production in cell free system. In vivo, CDE counteracted (P ≤ 0.0034) the effect of single EtOH administration which induced (P 2O2), free iron, and calcium levels while the CDE pre-treatment reversed increased (P ≤ 0.0051) all these intracellular disturbances. Conclusions: These findings suggest that CDE inhibits neutrophil ROS production and protects against EtOH-induced haematologiacal parameters changes and erythrocytes oxidative stress. The haematoprotection offered by chamomile might involve in part its antioxidant properties as well as its opposite effect on some intracellular mediators such as H2O2, free iron, and calcium.

GERMAN chamomile; NEUTROPHILS; ETHANOL; OXIDATIVE stress; CYTOCHROME c; ERYTHROCYTES

Lipids in Health & Disease, 2016, Vol 15, p1

1476-511X

Academic Journal

10.1186/s12944-016-0233-4