miR-320a affects spinal cord edema through negatively regulating aquaporin-1 of blood-spinal cord barrier during bimodal stage after ischemia reperfusion injury in rats.

Xiao-Qian Li; Bo Fang; Wen-Fei Tan; Zhi-Lin Wang; Xi-Jia Sun; Zai-Li Zhang; Hong Ma; Li, Xiao-Qian; Fang, Bo; Tan, Wen-Fei; Wang, Zhi-Lin; Sun, Xi-Jia; Zhang, Zai-Li; Ma, Hong

Background: Spinal cord edema is a serious complication and pathophysiological change after ischemia reperfusion (IR) injury. It has been demonstrated closely associated with bimodal disruption of blood-spinal cord barrier (BSCB) in our previous work. Aquaporin (AQP)1 plays important but contradictory roles in water homeostasis. Recently, microRNAs (miRs) effectively regulate numerous target mRNAs during ischemia. However, whether miRs are able to protect against dimodal disruption of BSCB by regulating perivascular AQP1 remains to be elucidated.Results: Spinal water content and EB extravasation were suggested as a bimodal distribution in directly proportion to AQP1, since all maximal changes were detected at 12 and 48 h after reperfusion. Further TEM and double immunofluorescence showed that former disruption of BSCB at 12 h was attributed to cytotoxic edema by up-regulated AQP1 expressions in astrocytes, whereas the latter at 48 h was mixed with vasogenic edema with both endothelial cells and astrocytes involvement. Microarray analysis revealed that at 12 h post-injury, ten miRs were upregulated (>2.0 fold) and seven miRs were downregulated (Conclusion: These findings indicate miR-320a directly and functionally affects spinal cord edema through negatively regulating AQP1 of BSCB after IR.

REPERFUSION injury; AQUAPORINS; MICRORNA; LABORATORY rats; IMMUNOFLUORESCENCE; CELL metabolism; RNA metabolism; ANIMAL experimentation; CAPILLARY permeability; EDEMA; EPITHELIAL cells; ISCHEMIA; MEMBRANE proteins; RATS; RNA; SPINAL cord

BMC Neuroscience, 2016, Vol 17, p1

1471-2202

Academic Journal

10.1186/s12868-016-0243-1