HIGH AVIDITY ANTI-ß2-GLYCOPROTEIN I ANTIBODIES ACTIVATE HUMAN CORONARY ARTERY ENDOTHELIAL CELLS AND TRIGGER PERIPHERAL BLOOD MONONUCLEAR CELL MIGRATION.

ARTENJAK, A.; KOZELJ, M.; LAKOTA, K.; CUCNIK, S.; BOZIC, B.; SODIN-SEMRL, S.

Anti-ß2-glycoprotein I antibodies (aß2GPI) represent a potential pathogenic candidate for coronary artery diseases. High avidity aß2GPI (HAv aß2GPI) are known to be associated with thrombotic and obstetric manifestations in patients with antiphospholipid syndrome, who are also susceptible to the development of premature atherosclerosis. However, there is little information about how human coronary artery endothelial cells (HCAEC) are affected by HAv aß2GPI. The purpose of our study was to evaluate the pathophysiological effects of HAv aß2GPI on HCAEC and determine their influence on cytokine expression and migration of peripheral blood mononuclear cells. Following the two hit hypothesis, we co-stimulated HAv aß2GPI-treated HCAEC in the presence and absence of the acute phase protein serum amyloid A (SAA). HAv aß2GPI induced in vitro HCAEC dysfunction, through the ERK1/2 signaling pathway, promoted the expression of chemokines (MCP-1, GROa and IL-8) and IL-6, which led to the attraction and migration of peripheral blood mononuclear cells. These effects were potentiated and intensified in conditions with SAA, indicating that HAv aß2GPI, in the presence of physiological concentrations of acute-phase proteins represent pathogenic autoantibodies, which could lead to the development of premature atherosclerosis and/or thrombosis development.

CORONARY artery physiology; GLYCOPROTEINS; IMMUNOGLOBULINS; ENDOTHELIAL cells; PHYSIOLOGICAL effects of cytokines; CHEMOKINES; ATHEROSCLEROSIS treatment

European Journal of Inflammation, 2013, Vol 11, Issue 2, p385

1721-727X

Academic Journal

10.1177/1721727X1301100209