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- Title
Amniotic membrane allografts maintain key biological properties post SCCO<sub>2</sub> and lyophilization processing.
- Authors
McDaniel, Jennifer S; Wehmeyer, Jennifer L; Cornell, Lauren E; Johnson, Anthony J; Zamora, David O
- Abstract
Amniotic membrane (AM) has been shown to enhance corneal wound healing due to the abundance of growth factors, cytokines, and extracellular matrix (ECM) proteins inherent to the tissue. As such, AM has garnered widespread clinical utility as a biological dressing for a number of ophthalmic and soft tissue applications. The preparation, sterilization, and storage procedures used to manufacture AM grafts are extremely important for the conservation of inherent biological components within the membrane. Current processing techniques use harsh chemicals and sterilization agents that can compromise the fundamental wound healing properties of AM. Furthermore, commercially available cryopreserved AM products require specific storage conditions (e.g., ultra-low freezers) thereby limiting their clinical availability in austere environments. Supercritical carbon dioxide (SCCO2) technology allows for the sterilization of biological tissues without the resulting degradation of integral ECM proteins and other factors often seen with current tissue sterilization processes. With this study we demonstrate that lyophilized AM, sterilized using SCCO2, maintains similar biochemical properties and biocompatibility as that of commercially available AM products requiring specialized cold storage conditions.
- Subjects
AMNION; SUPERCRITICAL carbon dioxide; FREEZE-drying; BIOLOGICAL dressings; CORNEA injuries; COLD storage; WOUND healing
- Publication
Journal of Biomaterials Applications, 2021, Vol 35, Issue 6, p592
- ISSN
0885-3282
- Publication type
Academic Journal
- DOI
10.1177/0885328220952585