Isolation of neoantigen-specific T cells from tumor and peripheral lymphocytes.

Cohen, Cyrille J.; Gartner, Jared J.; Horovitz-Fried, Miryam; Shamalov, Katerina; Trebska-McGowan, Kasia; Bliskovsky, Valery V.; Parkhurst, Maria R.; Ankri, Chen; Prickett, Todd. D.; Crystal, Jessica S.; Yong F. Li; El-Gamil, Mona; Rosenberg, Steven A.; Robbins, Paul F.; Li, Yong F

Adoptively transferred tumor-infiltrating T lymphocytes (TILs) that mediate complete regression of metastatic melanoma have been shown to recognize mutated epitopes expressed by autologous tumors. Here, in an attempt to develop a strategy for facilitating the isolation, expansion, and study of mutated antigen-specific T cells, we performed whole-exome sequencing on matched tumor and normal DNA isolated from 8 patients with metastatic melanoma. Candidate mutated epitopes were identified using a peptide-MHC-binding algorithm, and these epitopes were synthesized and used to generate panels of MHC tetramers that were evaluated for binding to tumor digests and cultured TILs used for the treatment of patients. This strategy resulted in the identification of 9 mutated epitopes from 5 of the 8 patients tested. Cells reactive with 8 of the 9 epitopes could be isolated from autologous peripheral blood, where they were detected at frequencies that were estimated to range between 0.4% and 0.002%. To the best of our knowledge, this represents the first demonstration of the successful isolation of mutation-reactive T cells from patients' peripheral blood prior to immune therapy, potentially providing the basis for designing personalized immunotherapies to treat patients with advanced cancer.

ALGORITHMS; AMINO acids; ANTIGEN-antibody reactions; ANTIGENS; CELL culture; CELL receptors; DNA; DOCUMENTATION; GENES; GENOMES; IMMUNITY; LYMPHOCYTES; MELANOMA; GENETIC mutation; PEPTIDES; PROTEINS; RESEARCH funding; RNA; T cells; TRANSCRIPTION factors; TUMOR antigens; HLA-B27 antigen; DNA-binding proteins; NUCLEAR proteins; INTERFERON gamma release tests

Journal of Clinical Investigation, 2015, Vol 125, Issue 10, p3981

0021-9738

Academic Journal

10.1172/JCI82416