Generation of IgE-specific cytotoxic T lymphocytes as a novel immunotherapeutic approach for the treatment of allergic asthma.

Kangle Cui; Yanna Chen; Li Zhou; Chunxia Yang; Yao Du; Weixing Shi; Zeling Cai; Jian Chen; Cui, Kangle; Chen, Yanna; Zhou, Li; Yang, Chunxia; Du, Yao; Shi, Weixing; Cai, Zeling; Chen, Jian

Introduction: Overproduction of immunoglobulin E (IgE) by a subset of B cells plays a key role in the pathogenesis of allergic asthma. Anti-IgE monoclonal antibodies have been successfully used to treat the disease, but long-term application is required.Methods: For this study, cytotoxic T lymphocytes (CTLs) against IgE-producing B cells were generated ex vivo by stimulating naive CD8 T cells with IgE-derived peptides presented by Drosophila-derived artificial antigen-presenting cells. Based on the treatment of allergic asthma in mice, the inhibitive effect of this CTL on IgE responses and airway inflammation was determined with the enzyme-linked immunosorbent assay and histochemical method.Results: The IgE-specific CTLs effectively lysed target cells in vitro, while the adoptively transferred CTLs specifically inhibited IgE responses and airway inflammation in an asthmatic mouse model. The effect of IgE-specific CTLs is MHC (major histocompatibility complex) Class I-restricted and requires the expression of perforin.Conclusion: IgE-specific CTLs generated ex vivo may provide a novel treatment for allergic asthma and lead to a new therapy for other immunological disorders.

CYTOTOXIC T cells; IMMUNOGLOBULIN E; ARTIFICIAL cells; PATHOLOGY; B cells; MAJOR histocompatibility complex; ASTHMA treatment; IN vitro studies; BIOLOGICAL models; IMMUNOGLOBULINS; ANIMAL experimentation; T cells; ALLERGIES; IMMUNOTHERAPY; MICE

Singapore Medical Journal, 2020, Vol 61, Issue 4, p212

0037-5675

Academic Journal

10.11622/smedj.2019068