EBSCO Logo
Connecting you to content on EBSCOhost
Results
Title

Human Epidermal Keratinocytes in Culture: A Story of Multiple Recipes for a Single Cell Type.

Authors

Poumay, Yves; Faway, Emilie

Abstract

Background: For one half-century, cultures of human epidermal keratinocytes have opened new paths of research in skin biology and dermatology. Either performed with serum and feeder layer, in serum-free conditions, or in autocrine conditions, cells cultured as monolayers became research materials for basic science and dermatology, as well as a source for grafting, particularly to treat severely burned patients. More recently, tissue reconstruction at air-liquid interface has opened new perspectives for in vitro toxicology, studies of epidermal barrier, and modeling skin diseases. Summary: This review presents a brief retrospective of the emergence of keratinocyte-based culture techniques. It also presents opportunities and eventual problems that researchers might encounter when exploring the skin using such procedures. Key Messages: While methodologies in tissue culture evolve, the multiplicity of procedures concomitantly increases, requiring to make some selective but difficult choice. Keeping tracks of technological evolution in epidermal cell culture should help choosing the adequate methodology for a specific investigation or innovating with new, more dedicated ones.

Subjects

KERATINOCYTES; CELLULAR evolution; CELL culture; SKIN diseases; RESEARCH personnel; KERATINOCYTE differentiation

Publication

Skin Pharmacology & Physiology, 2023, Vol 36, Issue 5, p215

ISSN

1660-5527

Publication type

Academic Journal

DOI

10.1159/000534137

EBSCO Connect | Privacy policy | Terms of use | Copyright | Manage my cookies
Journals | Subjects | Sitemap
© 2025 EBSCO Industries, Inc. All rights reserved