Prevention of Cutaneous Penetration and CD1c Uptake of Pollen Allergens by a Barrier-Enhancing Formulation.

Meinke, Martina Claudia; Schanzer, Sabine; Richter, Heike; Rippke, Frank; Filbry, alexander; Bohnsack, Kerstin; Patzelt, alexa; Lademann, Jürgen

Recent studies have shown that pollen proteins can penetrate the impaired skin barrier of atopic patients and exacerbate their disease. In the presented study the effect of a topically applied barrier-enhancing formulation was investigated for its preventive effect on the uptake of pollen allergens into CD1c+ epidermal cells. The pollen proteins were fluorescence labelled and applied on barrier-disrupted excised human skin. CD1c+ cells were selected after magnetic cell sorting and analysed using laser scanning microscopy. In untreated disrupted skin, 81% of the CD1c+ cells contained the fluorescence-labelled pollen allergens. In formulation-pretreated skin only 12% of the CD1c+ cells showed an uptake of pollen allergens. These results encourage the treatment of atopic patients with barrier-enhancing formulations to reduce the impact of pollen on air-exposed skin areas and hence the exacerbation of cutaneous symptoms.

ATOPIC dermatitis; ATOPIC dermatitis treatment; CD1 antigen; POLLEN; ALLERGENS; SKIN inflammation; PATIENTS

Skin Pharmacology & Physiology, 2016, Vol 29, Issue 2, p71

1660-5527

Academic Journal

10.1159/000444717