Pitfalls and Technical Aspects during the Research of Intestinal Anastomotic Healing in Rats.

Marjanovic, G.; Holzner, P.; Kulemann, B.; Kuesters, S.; Karcz, W. K.; Timme, S.; zur Hausen, A.; Baumann, T.; Hopt, U.T.; Obermaier, R.; Hoeppner, J.

Background: Fundamental experimental research into intestinal anastomotic healing in rodent models will gain increasing interest in the future. Methods: The aim of this study was to describe our 5-year experience with a standardized experimental setup of small and large bowel anastomoses in a rodent model and present a basic set of assessment tools investigating anastomotic healing. Anastomotic technique, perioperative complications such as anastomotic insufficiency (AI) and obstructive ileus were in the focus. Results: During different studies with varying study patterns, 167 rat small bowel anastomoses and 120 colonic anastomoses were performed. Overall mortality was 3.6% in small bowel and 2.5% in colonic anastomoses, AI occurred in 2.9 and 4%, respectively. A postoperative obstructive ileus was seen in 3/167 small bowel anastomoses and none in the colonic group. Conclusion: When performing experimental intestinal anastomoses in a standardized operative setting and critically considering special perioperative issues, the incidence of relevant complications can be maintained at an adequately low level. Copyright © 2010 S. Karger AG, Basel

SURGICAL anastomosis; WOUND healing; INTESTINAL injuries; LABORATORY rats; ANIMAL models in research; LARGE intestine surgery; COLECTOMY; THERAPEUTICS

European Surgical Research, 2010, Vol 45, Issue 3/4, p314

0014-312X

Academic Journal

10.1159/000320768